Program Overview

 

In partnership with MCCC:

  1. Introduction to Biotechnology (BIT 120)
  • Fall semester (Sept. – Dec.) (4 credits)
  1. Techniques & Instrumentation for Biotechnology (BIT 123)

  • Spring semester (Jan. – May) (4 credits)

 

What Do We Do in BIT 120?

Theory

1)Overview of DNA-RNA-Protein

2)How to make a protein

3)Fields in Biotechnology (with an emphasis on Medical Research)

1)Medical Biotechnology

2)Bioremediation

3)Forensics

4)Plant Biotechnology. etc

 

Laboratory Applications

1)Using Pipets

2)Isolation of DNA from students

3)Isolation of Bacteria from school surroundings

4)Gram Staining of Unknown Bacteria

5)Recombinant DNA Technology

 - Transformation of GFP gene into bacteria

 - Purification of GFP Protein

 

What Do We Do in BIT 123?

Laboratory Applications

1)Preparation and pH of solutions

2)CRISPR -Cos9 gene editing

3)Plasmid Purification

4)DNA Quantitation/Purity via Spectrophotometer

5)Agarose Gel Electrophoresis

6)Protein Purification

7)Protein Quantitation/Standard Curves

8)Polyacrylamide Gel Electrophoresis/Coomasie Blue

9)Forensics PCR – “Who dunnit?”

10) ELISAs – simulated virus outbreak

11) Mammalian Cell Culture

 

Key Skills:

  • Pipetting
  • Recombinant DNA
  • Protein Purification
  • PCR
  • Spectrophotometry
  • Immunology
  • Mammalian Cell Culture

     

Program Admission Requirements:

  • 12th grade preferred
  • Successful completion of Honors Science and Math courses (≥ B)
  • Prerequisites: Chemistry, Biology, Algebra 1 & 2
  • Concurrent or Prior: AP Biology
  • Strong interest in science fields and desire to pursue in college